HiSeq 2500

HiSeq 2500 system is a new Illumina sequencer for large-scale Next Generation Sequencing projects. Especially in combination with the SBS v4 chemistry, the HiSeq 2500 is extremely powerful. We were one of the first to embrace the NGS technology with the Solexa. Over time we provided services on the GAIIx, the HiSeq 2000, and now acquired the new Illumina HiSeq 2500 System. We offer NGS applications with sample preparation and advanced bioinformatics services.

This platform offers the ideal combination of a high output with low error rate. Read more about our Next Generation Sequencing services or contact us now for a NGS service quotation


Illumina HiSeq 2500 Advantages:

The HiSeq 2500 platform is compatible with the newest HiSeq v4 SBS reagents of Illumina. The combination of the new chemistry and the factory build sequencer allows for a fast turnaround time, longer reads, more data output and a higher quality.

  • Read Lengths: Paired End 2x125bp read lengths
  • Quality Score >80% Q30 
  • Data output >500 Gb per flow cell
  • Sequencing time shortened by 50%


HiSeq 2500 with the v4 Reagents:

What makes the Illumina sequencing technology so powerful is the combination of high output, together with a low error rate. By using a reversible terminator-based method, single bases are detected as they are incorporated into growing DNA strands (sequencing-by-synthesis). 

The new HiSeq v4 chemistry supports massively parallel sequencing increasing the throughput with 33% compared to the TruSeq technology. The HiSeq 2500 can generate up to 500 Gb data per flow cell with the v4 reagents. The v4 reagents is only compatible with the factory build version of the HiSeq 2500.


NGS Applications:

Hiseq 2500 technology supports both single read and paired-end libraries with different read lengths up to 2x125bp. The platform enables diverse DNA sequencing applications such as genome analysis (de novo and resequencing for variant calling), whole exome sequencing, metagenomic sequencing, candidate region targeted sequencing, sequencing amplicons / PCR pools, ChIP-seq and more.

For RNA sequencing, we offer mRNA seq for transcriptome analysis, sequencing-based differential expression analysis, small and microRNA quantification, and even DeepCAGE sequencing. Continue reading about our NGS services or download our Illumina sequencing guidelines.


Contact us for more information and unbiased and free project consultancy on what platform or application suits your project best.