The Next Generation Sequencing technology enables sequencing of an entire genome within matter of weeks, at a fraction costs when using Sanger Sequencing technology. This new technology is based on "sequencing by synthesis" and makes it possible to determine the sequence of the genome or transcriptome of any organism: with or without a reference sequence.
ServiceXS offers services on the Illumina HiSeq2000 and has several machines our disposal. With the HiSeq2000, ServiceXS runs 2 flow cells at a time. One flowcell contains 8 lanes, sometimes one of the lanes is used for an internal control. For multiplexing (more than one sample per lane) possibilities or for consultancy, please contact ServiceXS.
No. ServiceXS does not do RNA or DNA isolations, but we can advise you on isolation kits to use. We recommend the Macherey-Nagel RNA isolation kit. For customers in BeNeLux, ServiceXS recommends products supplied by BIOKÉ.
For our sample requirements and sample Quality Control (QC) policy, please download our service-specific guidelines.
ServiceXS can either do the sample preparation for you, or you can construct your own library. When you choose to do it yourself, please discuss this with your Project Manager at ServiceXS for an optimal Next Generation Sequencing (NGS) run.
Yes, ServiceXS does not require the whole flow cell to be filled. A consequence of only several independent lanes is that ServiceXS has to wait for another project to fill up the flow cell in order to be cost effective. This can cause delay in performance of the experiments. Do note that eventhough you may share a flow cell with another customer, we keep all data confidential.
We can either deliver raw data or offer you bioinformatic services. Do not underestimate the complexity of data generated from NGS! If you do not use our data analysis services, please make sure your bioinfomaticians are familiar and experienced with the vast amount of data generated.