Idaho Technology

Hi-Res Melting™ Curve Analysis

Melting Curve Analysis (MCA) is a simple technique that uses the decreasing fluorescence of a melting double-stranded DNA molecule to scan for the presence of DNA sequence variants (SNPs and mutations).

ServiceXS offers access to Hi-Res Melting™ technology (recently developed by Idaho Technology, Inc.), using the LightScanner Instrument.

Applications:

Pre-sequence scanning
SNP discovery & mutation detection
Genotyping by amplicon melting
Genotyping with unlabeled probes

Hi-Res Melting™ Advantages:

No Post-PCR processing
Excellent sensitivity & specificity
Detection of both heterozygous & homozygous variants
Flexible: 1, 96 or 384 samples per run
High-throughput
Low cost
Saves your sequencing budget

More information?

Download the Hi-Res Melting™ Brochure (PDF) or contact us for more information!